Marine eukaryotes and bacteria, Antarctica

Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm. 18S rDNA sequence information was obtained for representatives of each DGGE (denaturing gradient gel electrophoresis) band at each site and depth. This data was interrogated to phyla level: cnidaria, bryozoa, protozoa, dinoflagellates, arthropods, platyhelminths and annelids were present. Density of diatoms observed by fluorescence microscopy for each site and depth were recorded (per 100 ┬Ám2). At least 6 different diatom morphotypes were identified based on size and morphology. The slides were also examined by performing fluorescence in situ hybridization (FISH) reactions with bacteria-specific probes (Eub338), an archaea-specific probe (ARCH915) and 9 different bacterial group-specific probes. Bacterial groups identified from the sequences were: Gammaproteobacteria, SRB, Planctomycetales, Alphaproteobacteria, Archaea, Betaproteobacteria, Actinobacteria and Firmicutes, Cytophaga /Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia. This study was the first to examine eukaryotic recruitment, potentially overlooked in visual surveys, using molecular techniques. To use molecular techniques to examine the community structure of marine eukaryotes and bacteria recruiting onto artificial substrata.

Data collected during these periods: 01 Oct 2001 to 31 Oct 2002

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Data Cite
  • Australian Institute of Marine Science (AIMS). 2017, Marine eukaryotes and bacteria, Antarctica, http://data.aims.gov.au/metadataviewer/faces/view.xhtml?uuid=db3eae60-19b1-48bd-96a2-22bf4dd3ce47, accessed 22/06/2017
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  • Legal Access Constraint: intellectualPropertyRights
  • Legal Use Constraint: intellectualPropertyRights
  • Legal Other Constraint: All users of AIMS metadata must acknowledge the source of the material in the following manner: "Metadata was sourced from the Australian Institute of Marine Science (AIMS)." Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of references is as follows: "Australian Institute of Marine Science. [year-of-metadata-download], [Title], [metadata-access-URL], accessed [date-of-access]".
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Credits
Webster, NS: AIMS (Principal Investigator)
Battershill, CN: AIMS
Negri, AP: AIMS
Research was supported by Antarctica New Zealand, University of Canterbury, and a NZ Foundation for Research Science and Technology Postdoctoral Scholarship.
Thumbnail Image: Google Earth Mapping Service
Cited Responsible Party List
Principal Investigator
Webster, Nicole S, Dr Australian Institute of Marine Science (AIMS)
Point Of Contact List
Point Of Contact
Data Manager, AIMS Data Centre Australian Institute of Marine Science (AIMS) adc@aims.gov.au